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x box binding protein 1  (Proteintech)


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    Proteintech x box binding protein 1
    X Box Binding Protein 1, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/x+box+binding+protein+1/XBP1S-specific+Antibody/pm41212905-189-56-64
    Average 95 stars, based on 102 article reviews
    x box binding protein 1 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Western Blot:

    Article Title: DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway.
    Article Snippet: Protein bands were visualized using a chemiluminescence imaging system (Tanno5200) along with the SuperPico Enhanced chemiluminescence (ECL) Chemiluminescence Kit (#E422-C1-P1, Vazyme, China). .. The antibodies used for Western blot analysis included β-actin (#66009-1-Ig, Proteintech, USA), glyceraldehyde-3-phosphate dehydrogenase(GAPDH,#60004-1-Ig, Proteintech, USA), DNAJC5 (#DF4011, Affinity, USA), BiP (#11587-1-AP, Proteintech, USA), IRE1α (#27528-1-AP, Proteintech, USA), phospho-IRE1α (Ser724)(p-IRE1α,#AF7150, Affinity, USA), XBP1u (#bs23973R, Bioss, China), the spliced form of X-box binding protein 1(XBP1s,#24868-1-AP, Proteintech, USA), PERK (#24390-1-AP, Proteintech, USA), activating transcription factor 4 (ATF4,#97038, Cell Signaling, USA), ATF6 (#AF6243, Beyotime, China), and LC3 (#14600-1-AP, Proteintech, USA), p62(#AF0279, Beyotime, China), Beclin-1(#11306-1-AP, Proteintech, USA). .. The magnetic beads and antibodies were pretreated according to the specified protocol (#HY-K0202, MCE, USA).

    Article Title: DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway
    Article Snippet: Protein bands were visualized using a chemiluminescence imaging system (Tanno5200) along with the SuperPico Enhanced chemiluminescence (ECL) Chemiluminescence Kit (#E422-C1-P1, Vazyme, China). .. The antibodies used for Western blot analysis included β-actin (#66009-1-Ig, Proteintech, USA), glyceraldehyde-3-phosphate dehydrogenase(GAPDH,#60004-1-Ig, Proteintech, USA), DNAJC5 (#DF4011, Affinity, USA), BiP (#11587-1-AP, Proteintech, USA), IRE1α (#27528-1-AP, Proteintech, USA), phospho-IRE1α (Ser724)(p-IRE1α,#AF7150, Affinity, USA), XBP1u (#bs23973R, Bioss, China), the spliced form of X-box binding protein 1(XBP1s,#24868-1-AP, Proteintech, USA), PERK (#24390-1-AP, Proteintech, USA), activating transcription factor 4 (ATF4,#97038, Cell Signaling, USA), ATF6 (#AF6243, Beyotime, China), and LC3 (#14600-1-AP, Proteintech, USA), p62(#AF0279, Beyotime, China), Beclin-1(#11306-1-AP, Proteintech, USA). .. The magnetic beads and antibodies were pretreated according to the specified protocol (#HY-K0202, MCE, USA).

    Article Title: Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3‐dependent PDGFB signaling
    Article Snippet: Band intensities were quantified with ImageJ software ( https://imagej.net/software/fiji/downloads ). β‐Actin was utilized as an internal control. .. The following antibodies were used for western blotting: PERK (1:1, 000, ab229912, Abcam), pPERK (1:1, 000, DF7576, Affinity Biosciences), eukaryotic translation initiation factor 2 alpha (EIF2α, 1:1, 000, ET7111‐34, HUABIO), phosphorylated EIF2α (pEIF2α, 1:1, 000, #3398, Cell Signaling Technology, Danvers, MA, USA), IRE1α (1:1, 000, ab37073, Abcam), phosphorylated IRE1α (pIRE1α, 1:1, 000, ab48187, Abcam), the spliced form of X‐box binding protein 1 (XBP1s, 1:1, 000, 24868‐1‐AP, Proteintech), activating transcription factor 6 (ATF6, 1:1, 000, ER1706‐34, HUABIO), phosphoserine aminotransferase 1 (PSAT1, 1:1, 000, 10501‐1‐AP, Proteintech), phosphoglycerate dehydrogenase (PHGDH, 1:1, 000, 14719‐1‐AP, Proteintech), methylenetetrahydrofolate reductase (MTHFR, 1:1, 000, ab203786, Abcam), MAT2A (1:1, 000, #84478, Cell Signaling Technology), ATF4 (1:1, 000, #11815, Cell Signaling Technology), PI3K (1:1, 000, #4249, Cell Signaling Technology), p‐AKT (1:1, 000, #4060, Cell Signaling Technology), t‐AKT (1:1, 000, #9272, Cell Signaling Technology), β‐actin (1:10, 000, EM21002, HUABIO). .. For histone lysine‐methylation analysis, histones were extracted using the EpiQuikTM Total Histone Extraction Kit (OP‐0006, Epigentek, Farmingdale, NY, USA) following the manufacturer's protocol.

    Article Title: Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3-dependent PDGFB signaling.
    Article Snippet: Band intensities were quantified with ImageJ software (https://imagej.net/software/fiji/downloads). β-Actin was utilized as an internal control. .. The following antibodies were used for western blotting: PERK (1:1, 000, ab229912, Abcam), pPERK (1:1, 000, DF7576, Affinity Biosciences), eukaryotic translation initiation factor 2 alpha (EIF2α, 1:1, 000, ET7111-34,HUABIO), phosphorylatedEIF2α (pEIF2α, 1:1, 000, #3398, Cell Signaling Technology, Danvers, MA, USA), IRE1α (1:1, 000, ab37073, Abcam), phosphorylated IRE1α (pIRE1α, 1:1, 000, ab48187, Abcam), the spliced form of X-box binding protein 1 (XBP1s, 1:1, 000, 24868-1-AP, Proteintech), activating transcription factor 6 (ATF6, 1:1, 000, ER1706-34, HUABIO), phosphoserine aminotransferase 1 (PSAT1, 1:1, 000, 10501-1-AP, Proteintech), phosphoglycerate dehydrogenase (PHGDH, 1:1, 000, 14719- 1-AP, Proteintech), methylenetetrahydrofolate reductase (MTHFR, 1:1, 000, ab203786, Abcam), MAT2A (1:1, 000, #84478, Cell Signaling Technology), ATF4 (1:1, 000, #11815, Cell Signaling Technology), PI3K (1:1, 000, #4249, Cell Signaling Technology), p-AKT (1:1, 000, #4060, Cell Signaling Technology), t-AKT (1:1, 000, #9272, Cell Signaling Technology), β-actin (1:10, 000, EM21002, HUABIO). .. For histone lysine-methylation analysis, histones were extracted using the EpiQuikTMTotal Histone ExtractionKit (OP-0006, Epigentek, Farmingdale, NY, USA) following the manufacturer’s protocol.

    Binding Assay:

    Article Title: DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway.
    Article Snippet: Protein bands were visualized using a chemiluminescence imaging system (Tanno5200) along with the SuperPico Enhanced chemiluminescence (ECL) Chemiluminescence Kit (#E422-C1-P1, Vazyme, China). .. The antibodies used for Western blot analysis included β-actin (#66009-1-Ig, Proteintech, USA), glyceraldehyde-3-phosphate dehydrogenase(GAPDH,#60004-1-Ig, Proteintech, USA), DNAJC5 (#DF4011, Affinity, USA), BiP (#11587-1-AP, Proteintech, USA), IRE1α (#27528-1-AP, Proteintech, USA), phospho-IRE1α (Ser724)(p-IRE1α,#AF7150, Affinity, USA), XBP1u (#bs23973R, Bioss, China), the spliced form of X-box binding protein 1(XBP1s,#24868-1-AP, Proteintech, USA), PERK (#24390-1-AP, Proteintech, USA), activating transcription factor 4 (ATF4,#97038, Cell Signaling, USA), ATF6 (#AF6243, Beyotime, China), and LC3 (#14600-1-AP, Proteintech, USA), p62(#AF0279, Beyotime, China), Beclin-1(#11306-1-AP, Proteintech, USA). .. The magnetic beads and antibodies were pretreated according to the specified protocol (#HY-K0202, MCE, USA).

    Article Title: ATF6 Alleviates Endothelial Inflammation Following Extended Hepatectomy Through Inhibition of TRIM10 / NF ‐ κB Signaling
    Article Snippet: .. The primary antibodies used were anti‐ATF4 (1:1000, 10835‐1‐AP, Proteintech, Wuhan, China), X‐box binding protein 1 (Xbp1, 1:1000, 24868‐1‐AP, Proteintech, Wuhan, China), glucose‐regulated protein 78 (GRP78, 1:1000, WL03157, Wanleibio, Shenyang, China), GRP94 (1:1000, 14700‐1‐AP, Proteintech, Wuhan, China), ATF6 (1:1000, 24169‐1‐AP, Proteintech, Wuhan, China), TRIM 10 (1:1000, bs‐9409R, Bioss, Beijing, China), nuclear factor‐κB (NF‐κB) p65 (1:1000, bs‐20160R, Bioss, Beijing, China), phospho‐NF‐κB p65 (1:1000, ser536, bs‐0982R, Bioss, Beijing, China), and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH, 1:5000, 60004‐1‐Ig, Proteintech, Wuhan, China). .. Immunoreactivity was visualized using secondary horseradish peroxidase‐conjugated rabbit (1:5000, SA00001‐2, Proteintech, Wuhan, China) or mouse (1:5000, SA00001‐1, Proteintech, Wuhan, China) antibodies, and ECL Western Blotting Substrate ( P10100 , NCM Biotech, Suzhou, China).

    Article Title: Serpina3c Mitigates Adipose Tissue Inflammation by Inhibiting the HIF1α-Mediated Endoplasmic Reticulum Overoxidation in Adipocytes.
    Article Snippet: .. The following primary antibodies were used in this study: Serpina3c (50375- RP01, Sino Biological), HIF1α (BF8002, Affinity Biosciences, Cincinnati, OH, USA), Ero1α (67416-1-Ig, Proteintech, Rosemont, IL, USA), protein disulfide isomerase family A member 3 (PDIA3; DF6230, Affinity Biosciences), PDIA4 (DF12114, Affinity Biosciences), glucose regulated protein 78 (GRP78; AF5366, Affinity Biosciences), C/EBP homologous protein (CHOP; AF6277, Affinity Biosciences), phosphorylated eukaryotic initiation factor 2α (p-eIF2α; AF3087, Affinity Biosciences), eIF2α (AF6087, Affinity Biosciences), activating transcription factor 6 (ATF6; DF6009, Affinity Biosciences), spliced X-box binding protein 1 (XBP1S; 24868-1-AP, Proteintech), phosphorylated c-Jun N-terminal kinase (p-JNK; AF3318, Affinity Biosciences), JNK (AF6318, Affinity Biosciences), phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2; AF1015, Affinity Biosciences), ERK (AF0155, Proteintech), p-P38 (28796-1-AP, Proteintech), P38 (14064-1-AP, Proteintech), α-tubulin (66031-1-Ig, Proteintech). ..

    Article Title: DNAJC5 promotes cisplatin resistance in epithelial ovarian cancer by autophagy induced by the BiP/IRE1α/XBP1 endoplasmic reticulum stress pathway
    Article Snippet: Protein bands were visualized using a chemiluminescence imaging system (Tanno5200) along with the SuperPico Enhanced chemiluminescence (ECL) Chemiluminescence Kit (#E422-C1-P1, Vazyme, China). .. The antibodies used for Western blot analysis included β-actin (#66009-1-Ig, Proteintech, USA), glyceraldehyde-3-phosphate dehydrogenase(GAPDH,#60004-1-Ig, Proteintech, USA), DNAJC5 (#DF4011, Affinity, USA), BiP (#11587-1-AP, Proteintech, USA), IRE1α (#27528-1-AP, Proteintech, USA), phospho-IRE1α (Ser724)(p-IRE1α,#AF7150, Affinity, USA), XBP1u (#bs23973R, Bioss, China), the spliced form of X-box binding protein 1(XBP1s,#24868-1-AP, Proteintech, USA), PERK (#24390-1-AP, Proteintech, USA), activating transcription factor 4 (ATF4,#97038, Cell Signaling, USA), ATF6 (#AF6243, Beyotime, China), and LC3 (#14600-1-AP, Proteintech, USA), p62(#AF0279, Beyotime, China), Beclin-1(#11306-1-AP, Proteintech, USA). .. The magnetic beads and antibodies were pretreated according to the specified protocol (#HY-K0202, MCE, USA).

    Article Title: PGE2 Released by Pancreatic Cancer Cells Undergoing ER Stress Transfers the Stress to DCs Impairing Their Immune Function
    Article Snippet: This study shows that pancreatic cancer cells undergoing cell death by valproic acid (VPA) treatment activated dendritic cells (DCs) more efficiently than those treated with trichostatin A (TSA), as demonstrated by CD86 and CD80 surface expression.. Surprisingly though, DCs cultured in the presence of supernatant derived from VPA-treated cancer cells showed a reduced allostimulatory capacity and an increased release of IL10 and IL8 cytokines in comparison with those exposed to TSA-treated cell culture supernatant.. Searching for molecular mechanisms leading to such differences, we found that VPA treatment dysregulated choline metabolism and triggered a stronger endoplasmic reticulum (ER) stress in pancreatic cancer cells than TSA, upregulating CCAAT/enhancer-binding protein homologous protein, and activated cyclooxygenase-2, thus promoting the release of prostaglandin (PG) E2.

    Article Title: Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3‐dependent PDGFB signaling
    Article Snippet: Band intensities were quantified with ImageJ software ( https://imagej.net/software/fiji/downloads ). β‐Actin was utilized as an internal control. .. The following antibodies were used for western blotting: PERK (1:1, 000, ab229912, Abcam), pPERK (1:1, 000, DF7576, Affinity Biosciences), eukaryotic translation initiation factor 2 alpha (EIF2α, 1:1, 000, ET7111‐34, HUABIO), phosphorylated EIF2α (pEIF2α, 1:1, 000, #3398, Cell Signaling Technology, Danvers, MA, USA), IRE1α (1:1, 000, ab37073, Abcam), phosphorylated IRE1α (pIRE1α, 1:1, 000, ab48187, Abcam), the spliced form of X‐box binding protein 1 (XBP1s, 1:1, 000, 24868‐1‐AP, Proteintech), activating transcription factor 6 (ATF6, 1:1, 000, ER1706‐34, HUABIO), phosphoserine aminotransferase 1 (PSAT1, 1:1, 000, 10501‐1‐AP, Proteintech), phosphoglycerate dehydrogenase (PHGDH, 1:1, 000, 14719‐1‐AP, Proteintech), methylenetetrahydrofolate reductase (MTHFR, 1:1, 000, ab203786, Abcam), MAT2A (1:1, 000, #84478, Cell Signaling Technology), ATF4 (1:1, 000, #11815, Cell Signaling Technology), PI3K (1:1, 000, #4249, Cell Signaling Technology), p‐AKT (1:1, 000, #4060, Cell Signaling Technology), t‐AKT (1:1, 000, #9272, Cell Signaling Technology), β‐actin (1:10, 000, EM21002, HUABIO). .. For histone lysine‐methylation analysis, histones were extracted using the EpiQuikTM Total Histone Extraction Kit (OP‐0006, Epigentek, Farmingdale, NY, USA) following the manufacturer's protocol.

    Article Title: Dihydroartemisinin alleviates steatosis and inflammation in nonalcoholic steatohepatitis by decreasing endoplasmic reticulum stress and oxidative stress.
    Article Snippet: Nonalcoholic steatohepatitis (NASH) is a severely inflammatory subtype of nonalcoholic fatty liver.. Endoplasmic reticulum stress (ERS) and oxidative stress (OS) cause metabolic abnormalities, promote liver steatosis and inflammation, and are central to the development of NASH.. Dihydroartemisinin (DHA) is a compound extracted from Artemisia annua that is often used in the treatment of malaria.

    Article Title: Unfolded protein response kinase PERK supports survival and metastasis of circulating tumor cell clusters via SAM synthesis and H3K4me3-dependent PDGFB signaling.
    Article Snippet: Band intensities were quantified with ImageJ software (https://imagej.net/software/fiji/downloads). β-Actin was utilized as an internal control. .. The following antibodies were used for western blotting: PERK (1:1, 000, ab229912, Abcam), pPERK (1:1, 000, DF7576, Affinity Biosciences), eukaryotic translation initiation factor 2 alpha (EIF2α, 1:1, 000, ET7111-34,HUABIO), phosphorylatedEIF2α (pEIF2α, 1:1, 000, #3398, Cell Signaling Technology, Danvers, MA, USA), IRE1α (1:1, 000, ab37073, Abcam), phosphorylated IRE1α (pIRE1α, 1:1, 000, ab48187, Abcam), the spliced form of X-box binding protein 1 (XBP1s, 1:1, 000, 24868-1-AP, Proteintech), activating transcription factor 6 (ATF6, 1:1, 000, ER1706-34, HUABIO), phosphoserine aminotransferase 1 (PSAT1, 1:1, 000, 10501-1-AP, Proteintech), phosphoglycerate dehydrogenase (PHGDH, 1:1, 000, 14719- 1-AP, Proteintech), methylenetetrahydrofolate reductase (MTHFR, 1:1, 000, ab203786, Abcam), MAT2A (1:1, 000, #84478, Cell Signaling Technology), ATF4 (1:1, 000, #11815, Cell Signaling Technology), PI3K (1:1, 000, #4249, Cell Signaling Technology), p-AKT (1:1, 000, #4060, Cell Signaling Technology), t-AKT (1:1, 000, #9272, Cell Signaling Technology), β-actin (1:10, 000, EM21002, HUABIO). .. For histone lysine-methylation analysis, histones were extracted using the EpiQuikTMTotal Histone ExtractionKit (OP-0006, Epigentek, Farmingdale, NY, USA) following the manufacturer’s protocol.



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    SEC-B induces UPR activation, leading to ER stress. A: Confocal microscopy of ER-Tracker Green-stained HUVECs after 4 and 24 h of treatment with 10 μM SEC-B. The scale bar represents 25 μm. B: TEM images also showed the disorganization and enlargement of ER membranes as indicated by the red arrow (the scale bar represents 0.5 μm). C and D: Western immunoblotting analysis and quantification of XBP-1s and ATF6 expression levels in HUVECs treated with 10 μM SEC-B. Values are the mean ± SD of the values obtained from five independent experiments. ∗∗∗ P < 0.001 versus untreated cells. E: Confocal images of GRP78 immunostaining in HUVECs after 24 h treatment with 10 μM SEC-B. The scale bar represents 20 μm. F: Aggresome formation detected by the ProteoStat® Aggresome Detection Kit after 24 h of 10 μM SEC-B treatment. The scale bar represents 25 μm. Quantitative analysis of positive fluorescent area per cell, in HUVECs treated with 10 μM SEC-B for 24 h. Data are expressed as mean ± SD (n = 3). ∗∗ P < 0.01 versus untreated cells.

    Journal: Journal of Lipid Research

    Article Title: S-nitrosylation contributes to ER stress and aggresome formation in secosterol-B-mediated endothelial dysfunction

    doi: 10.1016/j.jlr.2026.101017

    Figure Lengend Snippet: SEC-B induces UPR activation, leading to ER stress. A: Confocal microscopy of ER-Tracker Green-stained HUVECs after 4 and 24 h of treatment with 10 μM SEC-B. The scale bar represents 25 μm. B: TEM images also showed the disorganization and enlargement of ER membranes as indicated by the red arrow (the scale bar represents 0.5 μm). C and D: Western immunoblotting analysis and quantification of XBP-1s and ATF6 expression levels in HUVECs treated with 10 μM SEC-B. Values are the mean ± SD of the values obtained from five independent experiments. ∗∗∗ P < 0.001 versus untreated cells. E: Confocal images of GRP78 immunostaining in HUVECs after 24 h treatment with 10 μM SEC-B. The scale bar represents 20 μm. F: Aggresome formation detected by the ProteoStat® Aggresome Detection Kit after 24 h of 10 μM SEC-B treatment. The scale bar represents 25 μm. Quantitative analysis of positive fluorescent area per cell, in HUVECs treated with 10 μM SEC-B for 24 h. Data are expressed as mean ± SD (n = 3). ∗∗ P < 0.01 versus untreated cells.

    Article Snippet: Blots were probed with the following antibodies: anti-spliced X-box binding protein 1 (XBP-1s) (E9V3E, #40435), ATF6 (D4Z8V, #65880), BiP/GRP78 (C50B12, #3177), and protein disulfide isomerase (PDI) (#2446) from Cell Signaling Technology; anti-endothelial NOS (eNOS) (A1548) from ABclonal; and anti-NOS2 (N-20, sc-651) from Santa Cruz Biotechnology.

    Techniques: Activation Assay, Confocal Microscopy, Staining, Western Blot, Expressing, Immunostaining